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KMID : 0380620150470020246
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 2 p.246 ~ p.253
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Study of Lipoprotein Lipase Inhibitory Activity of Anti-obesity Herb Extracts
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Lee Sung-Mee
Kang Yun-Hwan
Kim Kyoung-Kon
Kim Tae-Woo
Choe Myeon
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Abstract
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In this study, we evaluated the lipoprotein lipase (LPL) inhibitory activity of 11 water extracts derived from Cinnamomum cassia Blume, Sarcodon aspratus, Cordyceps militaris, Crataegus pinnatifida Bunge, Corni fructus, Allium cepa, Coix lacryma-jobi, Plantago asiatica L., Lentinus edodes, Rosa rugosa, and Foeniculum fructus. The results of the LPL secretion and activity assay showed Sarcodon aspratus (NE) extract have an LPL secretion inhibitory acitivity. The cause of reduction in LPL secretion after NE treatment was investigated using molecular biology methods. NE treatment affected the LPL content in cells, but did not affect LPL mRNA expression. It also increased the mRNA expression level of sortilin-related receptor LDLR class A (SorLA), a receptor that induces endocytosis and intracellular trafficking of LPL. Finally, cell fractionation revealed that NE treatment induced the expression of CCAAT-enhancer-binding protein beta (C/ EBPβ), a SorLA transcription factor, in the nuclei of 3T3-L1 adipocytes. These results show that NE’s anti-obesity effect involves inhibition of LPL secretion through C/EBPβ-mediated induction of SorLA expression.
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KEYWORD
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Sarcodon aspratus, lipoprotein lipase, sortilin-related receptor, CCAAT-enhancer-binding protein beta (C/EBPβ), anti-obesity
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